Assessment of morphological characterization and genetic diversity of lime and lemon accessions using ISSR marker

Abstract Despite the fact that lime and lemon groups have been collected as germplasm in Indonesian Citrus Subtropical Research Institute, a limited amount of research has conducted on identification evaluation these species. This study aimed to evaluate morphological, anatomy, genetic polymorphism, relationships among 16 accessions based six ISSR markers. The was at Punten Experimental Garden, Batu, East Java, Indonesia from January December 2021 order describe morphology anatomy characteristics. results showed 109 DNA bands were considered for analysis, which 88.1% polymorphic. dendrogram separated into two main coefficient 0.62. first group divided three clusters, i.e. A, B, C consist introduced varieties, most local lemons, limes, respectively. second only single member ( Limo Kwit ). closest distances between Cai Kahuripan Lemon Jumbo (88.1%) Uereka with Lime Bearr (88.3%). In contrast, lowest ones Montaji Lemo (52.9%) Swanggi Jari Buda (51.2%). general, traits reflected existing clusters contributed differences their plants. It is assumed accession hybrid subgenus papeda, so it belong Cluster II. finding very useful breeding programs Indonesia.